Probing Molecular Diversity with Mass Spectrometry

Probing protein-protein interactions with mass spectrometry.

Probing marine natural product defenses with DESI-imaging mass spectrometry.

T o demonstrate that a toxin or other natural product serves as a predator or pathogen deterrent requires that (i) the defined compound in question have the requisite biological activity, (ii) the compound is present at levels capable of eliciting an effect in the target organism, (iii) there is a physical mechanism for the substance to interact with its target before death of the producer, and...

Probing the Electron Capture Dissociation Mass Spectrometry of Phosphopeptides with Traveling Wave Ion Mobility Spectrometry and Molecular Dynamics Simulations

Electron capture dissociation mass spectrometry offers several advantages for the analysis of peptides, most notably that backbone c and z fragments typically retain labile modifications such as phosphorylation. We have shown previously that, in some cases, the presence of phosphorylation has a deleterious effect on peptide sequence coverage, and hypothesized that intramolecular interactions in...

Probing Bunyavirus N protein oligomerisation using mass spectrometry

RATIONALE Bunyaviruses have become a major threat to both humans and livestock in Europe and the Americas. The nucleocapsid (N) protein of these viruses is key to the replication cycle and knowledge of the N oligomerisation state is central to understanding the viral lifecycle and for development of therapeutic strategies. METHODS Bunyamwera virus and Schmallenberg virus N proteins (BUNV-N an...

Probing molecular associations of field-collected and laboratory- generated SOA with nano-DESI high-resolution mass spectrometry

[1] Aerosol samples from the 2010 CalNex field study in Bakersfield (BF) and Pasadena (LA) were analyzed using positive mode nanospray-desorption electrospray ionization mass spectrometry. Secondary organic aerosol (SOA) produced in a photochemical chamber by photooxidation of diesel (DSL) fuel and isoprene (ISO) under humid, high-NOx conditions, was analyzed for comparison. Three groups of org...